WebAug 1, 2008 · Each A domain (∼550 amino acids) recognizes a specific amino acid building block that it activates by adenylation and then transfers to the terminal thiol of the 4′-phosphopantetheine (Ppan) arm of the downstream PCP domain (∼80 amino acids). WebPhosphopantetheinyl transferases (PPTases) are a superfamily of essential enzymes required for the synthetic processes of many compounds including fatty acid, polyketide, and nonribosomal peptide metabolites.
Probing the Phosphopantetheine Arm Conformations of Acyl …
WebMar 8, 2024 · The enzyme phosphopantetheinyl transferase (PPTase) transforms an apo-ACP into holo-ACP by adding a 4′-phosphopantetheine (Ppant) moiety to a conserved serine residue of the ‘Asp-Ser-Leu’ (DSL) ... The Ppant arm linking conserved Ser 41 and Ser 36 position has been highlighted with an arrowhead. (B) LC-MS data confirm the formation of … WebJan 5, 2007 · The function of ACP in fatty acid biosynthesis is to shuttle the substrates attached to its phosphopantetheine arm as thioesters between the FAS enzymes. Analysis of the crystal structures of hexanoyl-, heptanoyl- and decanoyl-ACP reveals the details of how the protein stabilizes the growing fatty acyl chain by sequestering the aliphatic chain ... smail toyota
Decrypting the oscillating nature of the 4′‐phosphopantetheine arm …
WebOct 29, 2024 · Pantothenate kinase-associated neurodegeneration (PKAN) is an autosomal recessive movement disorder affecting children and adults. This profoundly disabling disorder manifests with severe, painful dystonia, young-onset parkinsonism, globus pallidus iron accumulation, and blindness from pigmentary retinopathy (Hayflick et al, 2003 ). WebJul 31, 2014 · (1) Movement of the ACP’s phosphopantetheine (Ppant) arm is thought to be coordinated with protein–protein interactions and significant rearrangement of the PKS enzyme assembly. (2-5) The dynamic structures of ACPs and their Ppant arms are difficult to track due to the flexibility of ACPs and ACPs’ transient interactions with catalytic … WebActivating Power of the Phosphopantetheine Arm in α-Oxoamine Synthase Catalysis Sarah E. Ackenhusen, Ye Wang, Stephanie W. Chun, Alison R. H. Narayan Abstract PLP … solicitation tracker