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WebMethods The mouse skeletal muscle myoblasts (C2C12) were induced to differentiate into myotubes, and then divided into groups as follows: control group (sterile PBS solution), LPS groups (1, 10... Web19 nov. 2024 · Following incubation for 6 h, the agent intervention was initiated. Preparation of LPS A total of 10 mg of LPS was dissolved in 10 ml of an aseptic PBS solution and gently shaken until completely dissolution. The LPS solution was vibrated for 30 min and was stored at − 20 °C. Web31 mei 2024 · The cyclic adenosine monophosphate (cAMP) agonist, forskolin (FSK), and the protein kinase A (PKA) inhibitor, HT89, were used to study the protective effect of LP … inmate medical center in kentucky prison