Irf reporter assay
WebProduct overview. IRF3 Transcription Factor Assay Kit (Colorimetric) (ab207210) is a high throughput assay to quantify IRF3 activation in nuclear extracts. This assay combines a … WebSep 15, 2005 · We developed a reporter assay system employing a self-inactivating retrovirus and analyzed the cystatin C and cathepsin C promoters. We found that a unique cis element mediates IRF-8-induced activation of both promoters.
Irf reporter assay
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WebHCT116-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1 binding sites. HCT116-Dual™ cells also … WebMar 29, 2024 · Dual-luciferase reporter assay Artificially synthesized SOX4 promoter sequence fragments were inserted into the pMIR-reporter (Promega) after enzyme digestion. Next, the correctly sequenced luciferase reporter plasmids were respectively cotransfected with LV-NC, LV-LIN00470, LV-EGR2, si-NC, and si-LINC00470 into U87 cells.
WebOct 14, 2024 · The anti-inflammatory activities of water extract and individual compounds were screened by NF-κB and interferon regulatory factor (IRF) reporter assays in THP-1 … WebTranscription factors of the interferon regulatory factor (IRF) family have been identified as critical mediators of early inflammatory gene transcription in infected cells. We recently determined that, besides IRF-3 and IRF-7, IRF-5 serves as a direct transducer of virus-mediated signaling.
WebAug 1, 2009 · IRF4 directly binds the Il10 gene as evidenced by chromatin immunoprecipitation assay, and regulates Il10 control elements in a reporter assay. IRF4 …
WebCustom DNA Oligos, RNAi & Assays. R&D Systems™ Luminex™ Assays Tool; Centrifuges & Microcentrifuges. Centrifuges & Microcentrifuges. Benchtop Centrifuges; Centrifugal Filter …
WebInvivoGen reporter cells are designed to monitor the activation of intracellular signaling pathways. These engineered cell lines are used for cell-based assays of cytokine, PRR, agonist or antagonists. These cells stably express … chips law and disorderWebNOTE: The IRF Provider Preview Reports will not be in your facility’s Validation Report (VR) folder. 5. Selecting the desired IRF Provider Preview report link from the list of reports … graphene market forecastWebDescription. THP1-Dual™ cells feature the Lucia gene, a secreted luciferase reporter gene, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated … graphene mark of the beastWebOr IECs were infected or mock-infected with PEDV for 12 h prior to transfection the luciferase reporter plasmids alone or cotransfection the indicated expression plasmids (0.5 μg). The cell lysates were harvested and luciferase activity was analyzed using a dual-luciferase assay system and a luminometer (Turner BioSystems, Inc. Sunnyvale, CA ... chips lawn careWebCell-Based Assays Luciferase Reporter Stable Cell... graphene materialsWebApr 11, 2016 · Established the manufacturing, chemistry, biochemistry and TC laboratories. Validated cell culture methods, reporter assays and lysate experiments on SF9, 3T3 and … chips lawn care temple gaWebRather, PLpro interacts with IRF-3 and inhibits the phosphorylation and nuclear translocation of IRF-3, thereby disrupting the activation of type I IFN responses through either Toll-like receptor 3 or retinoic acid-inducible gene I/melanoma differentiation-associated gene 5 pathways. ... activation inhibit and luciferase reporter assay: 1, 2, 3 ... chips law enforcement