High-throughput screens for eef-2 kinase
WebEnter the email address you signed up with and we'll email you a reset link. WebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput …
High-throughput screens for eef-2 kinase
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WebMay 20, 2024 · Background: Eukaryotic elongation factor 2 kinase (eEF2K) regulates the elongation stage of protein synthesis by phosphorylating eEF2, a process related to various diseases including cancer and cardiovascular and neurodegenerative diseases. In this study, we describe the identification of novel eEF2K inhibitors using high-throughput screening … WebIntroduced, optimized and conducted an in vitro high-throughput drug screen assay for compounds with activity against liver stage malaria. Discovered and confirmed several compounds active against ...
WebSep 18, 2024 · A high-throughput screen (kinase reaction performed at room temperature) revealed the compound TCMDS-135051 as a highly selective and specific Pf CLK3 kinase inhibitor. This was confirmed in vivo by the finding that parasite strains that developed resistance against TCMDS-135051 acquired mutations in Pf CLK3. WebNov 1, 2016 · Purified human eEF2K was obtained from an expression system and a luminescence-based high-throughput screening (HTS) assay was developed using MH-1 …
WebJul 15, 2014 · Eukaryotic elongation factor-2 kinase (eEF2K), encoded by the EEF2K gene, is well-known to be a Ca 2+ /calmodulin (CaM)-dependent kinase which can negatively modulate protein synthesis. It is highly conserved among eukaryotes from mammals to invertebrates, of which human and mouse may have 99 % overall amino acid identity. WebMay 21, 2016 · Search worldwide, life-sciences literature Search. Advanced Search
WebAug 5, 2015 · High-throughput screens for eEF-2 kinase. Journal of Biomolecular Screening 19: 445–452. Article CAS PubMed Google Scholar Drews, J. 2000. Drug discovery: A historical perspective. Science 287: 1960–1964. Article CAS PubMed Google Scholar Du, Y. 2015. Fluorescence polarization assay to quantify protein-protein interactions in an HTS …
WebeEF-2 kinase is a highly conserved protein kinase in the calmodulin -mediated signaling pathway that links multiple up-stream signals to the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 ( EEF2) and thus inhibits the EEF2 function. [6] [8] Activation [ edit] can a clock be overwoundWebHigh-throughput screens for eEF-2 kinase (Q35002039) From Wikidata. Jump to navigation Jump to search. scientific article. edit. Language Label Description Also known as; English: High-throughput screens for eEF-2 kinase. scientific article. Statements. instance of. scholarly article. 1 reference. stated in. fish course recipesWebJun 10, 2014 · High-throughput screening platforms have proved beneficial for protein kinase drug discovery and development, as large compound libraries can be screened with ease and compound potency and selectivity across a subset of the kinome can be determined [13], [14]. can a clock just stop workingfish coveWebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput assay systems may expedite the process. Two high-throughput assays are described for eEF-2K using recombinant, tag-free enzyme purified from bacteria. fish cove bayberryWebMay 1, 2014 · The activity of eEF-2, the main eEF involved in the translocation step of elongation, can be inhibited by the eukaryotic elongation factor 2 kinase (eEF2K) (Ma, 2024). eEF2K is an... can a clock spring be repairedWebHere, we describe the purification of the protein kinase, eEF-2K, and the utilization of three biochemical assays in the course of identifying small molecules that block its enzymatic … fish cove bayberry plaza